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. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Nat Chem Biol. 2020 Oct 5;17(2):178–186. doi: 10.1038/s41589-020-00657-7

Extended Data Fig. 1. Ang-Tie2 complex formation occurs in a sulfation-dependent manner.

Extended Data Fig. 1

Binding of biotinylated HS sulfation motifs to immobilized Tie2 in the presence or absence of (a) Ang1 or (b) Ang4, as detected by ELISA. Removal of the N- and O-sulfate groups (De-N,O), the N-sulfate groups (De-N), the O-sulfate groups (De-O), the 6-O-sulfate groups (De-6O), or the 2-O-sulfate groups (De-2O), or conversion of the N-sulfate to N-acetyl groups (N-Ac) reduced formation of HS-Ang1/4-Tie2 ternary complexes. Data represent mean ± s.e.m., n = 2 independent replicates.