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. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Nat Chem Biol. 2020 Oct 5;17(2):178–186. doi: 10.1038/s41589-020-00657-7

Extended Data Fig. 2. Orphan receptor Tie1 binds to the CS-E motif.

Extended Data Fig. 2

(a) Chemical structure of the CS-E sulfation motif in CS polysaccharides. Interaction of CS-E and Tie1 by (b) ELISA, (c) glycan microarray, and (d) surface plasmon resonance. Dissociation constant for Tie1, as determined by ELISA: KD,app = 19.9 nM (10.5 to 53.5 nM); value represents mean (95% CI); graphed data represent n = 2 independent replicates. For glycan microarrays, data represent mean ± s.e.m., n = 10 individual spots per glycan concentration. Dissociation constant for Tie1, as determined by SPR: KD,app = 14.7 nM (14.6 to 14.8 nM); value represents mean (95% CI). SPR data were fit using a 1:1 Langmuir model shown in red.