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. 2021 Apr 7;10:e61453. doi: 10.7554/eLife.61453

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional
information
Cell line (Homo sapiens) SH-SY5Y National Centre for Cell Science, Pune, India https://www.nccs.res.in/index.php/Nationalrepos/Repocellines Neuroblastoma cell line
Recombinant DNA reagent H72F (plasmid) This paper Clone used to generate H72F mutant (page 21)
Recombinant DNA reagent H121F (plasmid) This paper Clone used to generate H72F mutant (page 21)
Recombinant DNA reagent I113T (plasmid) Genscript
Recombinant DNA reagent G37R (plasmid) Genscript
Recombinant DNA reagent G85R (plasmid) Genscript
Sequence-based reagent H72F_F Biotech Desk PCR primer cctcactttaatcctctatccagaaaattcggtgggccaaagg
Sequence-based reagent H72F_R Biotech Desk PCR primer cctttggcccaccgaattttctggatagaggattaaagtgagg
Sequence-based reagent H121F_F Biotech Desk PCR primer ggccgcacactggtggtctttgaaaaagcagatgactt
Sequence-based reagent H121F_R Biotech Desk PCR primer aagtcatctgctttttcaaagaccaccagtgtgcggcc
Commercial assay or kit QuickChange XL site directed mutagenesis kit Agilent Cat# 200516
Commercial assay or kit Vybrant MTT cell proliferation assay kit Invitrogen Cat# V-13154
Chemical compound, drug 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) Avanti Polar Lipids Inc. Cat# 850355
Chemical compound, drug 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) Avanti Polar Lipids Inc. Cat# 850725
Chemical compound, drug 1,2-Dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) Avanti Polar Lipids Inc. Cat# 840035
Chemical compound, drug Cardiolipin Avanti Polar Lipids Inc. Cat# 710335
Chemical compound, drug 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiIC-18(3)) Invitrogen Cat# D3911
Chemical compound, drug Isopropyl ß-D-1-thiogalactopyranoside (IPTG) Sigma Aldrich Cat# I6758
Chemical compound, drug Thioflavin T Merck Cat# T3516
Chemical compound, drug Calcein AM Merck Cat# 17783
Software, algorithm OPM server Orientations of Proteins in Membranes https://opm.phar.umich.edu/ppm_server
Software, algorithm AGGRESCAN AGGRESCAN http://bioinf.uab.es/aggrescan/
Software, algorithm Image J National Institutes of Health https://imagej.nih.gov/ij/
Other Alexa Fluor 488 C5 maleimide Invitrogen Cat# A10254