Table 2. The values of Stern–Volmer quenching constants for Trp 32 residue of all the protein variants in the absence (Ksv,M−1) and presence of DPPC SUVs (Ksvm, M−1) as obtained from acrylamide quenching for WT SOD1 and all the mutants including apo SOD1.
Proteins | Ksv | Ksvm | Ksv/Ksvm |
---|---|---|---|
WT SOD1 | 6.8 ± 0.1 | 5.7 ± 0.2 | 1.19 |
H121F | 8.0 ± 0.1 | 6.3 ± 0.1 | 1.26 |
H72F | 12.7 ± 0.3 | 7.0 ± 0.2 | 1.82 |
apo SOD1 | 14.3 ± 0.1 | 7.6 ± 0.2 | 1.88 |