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. 2021 Apr 19;10:e61983. doi: 10.7554/eLife.61983

Figure 8. Glycyrrhizin (GLZ) increases diethylnitrosamine (DEN)-induced liver tumour number in ROCK1nc mice.

(A) Timeline of the DEN-induced hepatocellular carcinoma (HCC) and GLZ treatment protocol. (B) The number of liver tumours in ROCK1nc mice 9 months after treatment with GLZ alone (n = 10 mice) or after HCC initiation with DEN alone (n = 14 mice) or with GLZ (n = 10 mice). Boxes indicate upper and lower quartiles and median, whiskers indicate 5–95% percentiles, pairwise Student’s t-test as indicated. (C) Numbers of lung metastases in ROCK1nc mice 9 months after treatment with GLZ alone (n = 10 mice) or after HCC initiation with DEN alone (n = 14 mice) or with GLZ (n = 10 mice). Boxes indicate upper and lower quartiles and median, whiskers indicate 5–95% percentiles, pairwise Student’s t-test as indicated; ns: not significant. (D) Schematic diagram depicting how acute acting neutrophils influence tumour development. ROCK1wt mice had fewer recruited neutrophils and less tissue damage than ROCK1nc mice 3 days after equivalent DEN treatment. The healthier ROCK1wt liver tissue enabled more DEN-mutated hepatocytes to initiate the HCC tumours that were observed 9 months later. Following GLZ administration, ROCK1nc mice resembled ROCK1wt mice with fewer neutrophils and less tissue damage acutely, and more HCC tumours 9 months later. Figure created with BioRender.com.

Figure 8.

Figure 8—figure supplement 1. Similar liver mass in ROCK1nc mice treated with diethylnitrosamine (DEN) and glycyrrhizin (GLZ).

Figure 8—figure supplement 1.

(A) Liver mass, (B) body mass and (C) liver to body mass ratios for ROCK1nc mice 9 months after treatment with GLZ alone (n = 10 mice) or following hepatocellular carcinoma (HCC) initiation with DEN alone (n = 14 mice) or with GLZ (n = 10 mice). Boxes indicate upper and lower quartiles and median, whiskers indicate 5–95% percentiles.