Video 1. Time lapse of apoptotic ROCKwt mouse embryo fibroblasts (MEFs).

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ROCK1wt MEFs were plated on 6-well glass-bottom dishes at a density of 2 × 10⁶ cells per well. Cells were serum-starved overnight prior to induction of apoptosis with tumour necrosis factor α (TNFα) (50 ng/mL) and cycloheximide (10 µg/mL) diluted in serum-free starvation medium. Differential interference contrast (DIC) time-lapse microscopy images were acquired with a 20× DIC objective using a Nikon TE 2000 microscope with a heated stage and 5% CO₂ gas line. Immediately after induction of apoptosis the dishes were transferred to the microscope and time-lapse images were taken each minute.