Table 1. Conservation of ROCK1 caspase cleavage site.
| Mammals | |
| Homo sapiens | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Chimp | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Gorilla | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Orangutan | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Gibbon | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Rhesus | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Crab-eating macaque | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Baboon | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Green monkey | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Marmoset | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Squirrel monkey | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Bushbaby | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Chinese tree shrew | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Squirrel | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Lesser Egyptian jerboa | QLRAKLLDLSDSTSVASFPSADETDGSLP |
| Prairie vole | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Chinese hamster | QLRAKLLDLSDSTSIASFPSADETDGNLP |
| Golden hamster | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Mouse | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Rat | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Naked mole-rat | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Guinea pig | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Chinchilla | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Brush-tailed rat | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Rabbit | QLRAKLSDLSDSTSVASFPSADETDGNLP |
| Pika | QLRAKLLDLSDSTSVASFPSTDEaDGNLP |
| Pig | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Alpaca | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Bactrian camel | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Dolphin | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Killer whale | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Tibetan antelope | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Cow | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Sheep | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Domestic goat | QLRAKLLDLSDSTSVASFPSADETDGNLS |
| Horse | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| White rhinoceros | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Cat | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Dog | QLRAKLLDLSDSTSIASFPSADETDGNLP |
| Ferret | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Panda | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Pacific walrus | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Weddell seal | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Black flying-fox | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Megabat | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| David’s myotis (bat) | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Microbat | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Big brown bat | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Hedgehog | QLRAKLLDLADSTSVASFPSADETDGNLP |
| Shrew | QLRSKLLDLSDSTSVASFPSADETDGTLP |
| Star-nosed mole | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Elephant | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Cape elephant shrew | QLRAKLLDLSDSTSVASFPSADETDGSLP |
| Manatee | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Cape golden mole | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Tenrec | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Aardvark | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Armadillo | QLRAKLLDLSDSTSVASFPSADETDGNLP |
| Opossum | QLRAKLLDLSDSTSVTSFPSADETDGNLP |
| Tasmanian devil | QLRAKLLDLSDSTSVTSFPSADETDGNLP |
| Wallaby | QLRSKLSDLLDSGSVASL-S-DETDGNLV |
| platypus | QLRRKILDLLDSTSVASLQ--DETDGNLS |
| Birds | |
| Saker falcon | QLRRKILDLLDSTSVASLQ--DETDGNLS |
| Peregrine falcon | QLRRKIMDLLDSTSVASLP--DEmDGNLP |
| Collared flycatcher | QLRRKIMDLLDSTSVASLP--DETDGNLS |
| White-throated sparrow | QLRRKIMDLLDSTSVASLP--DETDGNLS |
| Medium ground finch | QLRRKIMDLLDSTSVASLP--DETDGNLS |
| Zebra finch | QLRRKIMDLLDSTSVASLP--DETDGNLS |
| Tibetan ground jay | QLRRKILDLLDSTSVASLQ--DETDGNLS |
| Budgerigar | QLRRKILDLLDSTSVASLQ--DETDGNLS |
| Parrot | QLRRKILDLLDSTSVASLQ--DETDGNLS |
| Scarlet macaw | QLRRKILDLLDSTSVASLQ--DETDGNLS |
| Rock pigeon | QLRRKILDLLDSTSVASLP--DETDGNLS |
| Mallard duck | QLRRKILDLLDSTSVASLQ--DETDGNLS |
| Chicken | QLRRKILDLLDSTSVSSLQ--DEiDGNLS |
| Turkey | QLRRKILDLLDSTSVSSLQ--DEiDGNLS |
| Sarcopterygii | |
| American alligator | QLRSKILDLLDSTSVASLQ--DETDGNVS |
| Green seaturtle | QLRSKILDLLDSTSVASLQ--DETDGNLT |
| Painted turtle | QLRSKLLDLLDSTSVASLQ--DETDGNLT |
| Chinese softshell turtle | QLRSKILDLLDSASVASLQ--DETDGNLT |
| Spiny softshell turtle | QLRSKILDLLDSASVASLQ--DETDGNLT |
| Lizard | QLRSKILDLLDSTSVASLP--DETDGNIV |
| X. tropicalis | QLRARLADLLDSTSVASLQ--DdTDGIIG |
| Coelacanth | QLRAKLMDLLDNTSVASLQ--DEvDGNVI |
| Fish | |
| Tetraodon | QLREKLNDLLDNSSITSLQ--DETDSNIA |
| Fugu | QLREKLNDLLDNSSITSLQ--DETDSNIA |
| Yellowbelly pufferfish | QLREKLNDLLDNSSITSLQ--DETDSNIA |
| Nile tilapia | QLREKLNDLLDNSSVTSLQ--DETDSNIA |
| Princess of Burundi | QLREKLNDLLDNSSVTSLQ--DETDSNIA |
| Burton's mouthbreeder | QLREKLNDLLDNSSVTSLQ--DETDSNIA |
| Zebra mbuna | QLREKLNDLLDNSSVTSLQ--DETDSNIA |
| Pundamilia nyererei | QLREKLNDLLDNSSVTSLQ--DETDSNIA |
| Medaka | QLREKLNDMLENSSITSLQ--DETDSNIA |
| Southern platyfish | QLREKLSDLLENSSVTSLQ--DETDSNTA |
| Stickleback | QLREKLNDLLDTSSVTSLQ--DETDGNIA |
| Atlantic cod | QLREKLNDLLDSSSVTSLL--DETDGNLA |
| Zebrafish | QLREKLNDLLDNSSVTSLQ--DElDSNIA |
| Spotted gar | QLREKLNDLLDNSSVASLQ--DEaDGNIA |
Protein sequences orthologous to human ROCK1 were aligned in 93 vertebrate species using Multiz Alignment (Blanchette et al., 2004) in the UCSC Genome Browser. These include representative species from the major classes: 62 mammals, 14 birds, 8 Sarcopterygii and 14 fish. The conserved caspase cleavage site indicated with underlined bold typeface, with non-identical amino acids in lowercase.