Figure 1.

WIN inhibits U251 cell viability. WIN dose-dependently (a) and time-dependently (b) inhibited U251 cell viability. U251 cells seeded in 96-well plate were treated with 0-40 μM WIN for 48 h or cells were treated with 20 μM WIN for 0-48 h. Cell viability was detected by MTT assay. (c) Morphological changes. Cells after treatment were detected by microscope (magnification, × 200). All experiments were repeated three times. Bar with “^∗” or “^∗∗” indicates the significant difference at P < 0.05 or P < 0.01 level, respectively.