Table 2.
Locus name, expected size, annealing temperature, and sequence of the 10 selected microsatellite primers.
| Primer | Expected size [bp] | Annealing T [°C] | Forward primer | Reverse primer |
|---|---|---|---|---|
| CNLTs2 | 160-260 | 58.2 | CAGCAGGGAGAGAGAGGAGA | GGGGTCAAGTTATTGCTTAGAGAA |
| CNLTs5 | 190-310 | 57.2 | CCCAAAGTGATGCAAAAACA | TAGATAGAGACACAGACACACACA |
| CNLTs6 | 90-260 | 58.2 | AATTCGCAGCTGATCTACGC | CTCGTCGATATACGTGCAAAA |
| CNLTs19 | 180-300 | 58.2 | CATTTCTTGCTGCTGGATCA | AGTATGGTGGCCTTGGTGAG |
| CNLTs22 | 100-260 | 55.7 | CATGCTCGTTCAGAGTCCAA | GGGGGATCTAGGAGAGAGAGA |
| CNLTs24 | 135-180 | 55.7 | GAGAGCGGTTTTGTCCTACG | GGAATAGGGAGGCGAGGTAG |
| CNLTs25 | 150-315 | 57.2 | TTGGAATGAGATGGCATTTG | GAAGCGGGGTAAGATTTGAA |
| CNLTs458 | 130-250 | 59.1 | AACAAGAACCACACAACA | AAAGGAACCCACAGGGGTAA |
| CNLTs461 | 220-320 | 63.9 | GTCTTGATGGTGGCGGAATAG | TCATCATCCTGCTCGAATCA |
| CNLTs463 | 190-260 | 59.1 | TGCTAGGATGGTCCTGTTGAG | AGCACCAAATCCCTATGCAC |