Figure 3.

HNRNPC suppression inhibited the Ras/MAPK signaling pathway, leading to arrest of more cells at S phase and blocking of EMT in vitro. (A, B) Western blot to detect activation of the Ras/MAPK signaling pathway in Lv-NC Huh-7, Lv-HNRNPC Huh-7, Lv-NC Hep 3B, and Lv-HNRNPC Hep 3B cells. (C, D) Cell cycle analysis for the various cell groups mentioned above. (E, F) Western blot to detect c-Myc, CDK4, and cyclin E1 levels in the various cell groups. (G, H) Western blot to analyze Vimentin and E-Cadherin expression in various cell groups. Each test was performed independently in triplicate, and the results were presented as mean ± standard deviation (SD). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.