Fig. 4.

Flow cytometry of aortic lysates and whole blood demonstrates leukocyte extravasation into the aortic endothelium by noise and prevention by LysM⁺ cell ablation. Flow cytometry and representative plots in aortic lysates show an increase in count following noise exposure and a reduction in count in all leukocytes (a) as well as innate immune leukocytes (b). Specifically, monocytes (c) and macrophages (d) were reduced below baseline control counts and no increase was found in the aorta following both noise exposure and LysM⁺ cell ablation. e All immune cell subsets were found decreased in whole blood of noise-exposed CTR mice or in the DTX-treated groups. Data points are measurements from individual animals; one-way ANOVA with Tukey’s multiple comparison test (a–e). ^*P < 0.05 vs. Control; ^#P < 0.05 vs. Noise; ^$P < 0.05 vs. DTX, ⁺P < 0.05 vs. DTX + Noise