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Detection of retinal hypoxia. As a positive control, retinal hypoxia induced by CCAO in mice was detected with formation of pimonidazole (Hypoxyprobe) adducts in retinal lysates on nitrocellulose membranes (A). The fixed eyes were stained with Hypoxyprobe primary antibody (red) and GSL-1 (green) and dissected and flat mounted for microscopic photographs (B).
