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. 2021 Apr 30;12(5):424. doi: 10.1038/s41419-021-03715-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a MIR99AHG expression was detected by RNA chromogenic in situ hybridization (CISH) on a TMA cohort containing 74 paired LUAD tissues and adjacent normal tissues. Representative images of nontumor and tumor tissues are shown. MIR99AHG was downregulated in tumor tissues (P = 0.006). b–d MIR99AHG expression was downregulated in higer T stage (b), N stage (c) or TNM stage (d) in the TMA cohort. e Kaplan–Meier analysis of the overall survival in the TMA cohort based on MIR99AHG (HR = 0.334, P = 0.001). f Univariate (upper panel) and multivaraite (lower panel) analyses of overall survival in the TMA cohort by Cox regression analysis. g, h Kaplan–Meier analysis of the overall (g HR = 0.655, P = 0.004) and disease-free (h, HR = 0.713, P = 0.023) survival in the TMA cohort based on MIR99AHG. A two-tailed Student’s t-test was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars, SEM.