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. 2021 Apr 30;12(5):424. doi: 10.1038/s41419-021-03715-7

Fig. 3. MIR99AHG suppresses proliferation, migration, and invasion of H1299 cells in vitro.

Fig. 3

a and b MIR99AHG inhibited the proliferation abilities of H1299 cells, as shown by RTCA proliferation assays (a), EdU assays (b, upper panel), and colony formation assays (b, lower panel). c–e MIR99AHG inhibited the migration and invasion abilities of H1299 cells, as shown by RTCA migration assays (c), wound-healing assays (d), and transwell and Matrigel assays (e). f Cell apoptosis analyses of H1299 cells transfected as indicated. Cells were harvested for Annexin-V/d PI double staining followed by flow cytometry analysis. All experiments were repeated three times with similar results and images are representative of three independent experiments. A two-tailed Student’s t-test was used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars, SEM.