Fig. 7. Comparison of C-repeat binding factors (CBFs).

a Phylogenetic tree analysis of CBFs from four tea resources and Arabidopsis thaliana. HD: Huangdan, SCZ: Suchazao¹⁵, LJ43: Longjing 43¹⁷, DASZ: An ancient tea tree¹⁹. b Three AtCBFs arranged in tandem on chromosome 4 in Arabidopsis. The binding sites of AtCBF1 and AtCBF2 verified by DNA affinity purification sequencing (DAP-seq) are presented in the form of sequence logos⁴³. c Six CBFs arranged in tandem on chromosomes 1 and 6 in the HD genome. d Multisequence alignment of the conserved binding domains of CBFs from HD, DASZ, and Arabidopsis. The sequence logo reflects the conservation of the amino acid at each position. The yellow box represents the AP2 DNA-binding domain; the purple overlines indicate two signature sequences; the small red box represents the amino acid mutation of CBFs of HD compared to DASZ