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. 2021 Apr 30;12(5):432. doi: 10.1038/s41419-021-03709-5

Fig. 3. The time course of the effects of TDZD-8 or LiCl on the TGF-β1 induced TEC profibrogenic plasticity.

Fig. 3

A TKPT were treated with TGF-β1 (2 ng/ml) for 24 h following pretreatment with TDZD-8 (10 μmol/L) or LiCl (10 mmol/L) for 30 min. Representative phase-contrast micrographs were shown. Scale bar = 100 μm. B TKPT were treated with TGF-β1(2 ng/ml) for 12 h, 24 h, 48 h following pretreatment with TDZD-8 (10 μmol/L) or (D) LiCl (10 mmol/L) for 30 min. Cell lysates were prepared for immunoblot analysis for E-cadherin, vimentin, FN, PAI-1, CTGF, pH3, and GAPDH. Representative immunoblots were shown. C, E Densitometric analyses of the expression of E-cadherin, vimentin, FN, PAI-1, CTGF, and pH3, as normalized to the expression of GAPDH and expressed as fold changes relative to the control group based on immunoblot analysis. #P < 0.05 versus TGF-β1  group (n = 3, t-test).