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. 2021 Apr 30;12(5):432. doi: 10.1038/s41419-021-03709-5

Fig. 4. The permissive effect of GSK3β on the TGF-β1 induced TEC profibrogenic plasticity.

Fig. 4

TKPT were subjected to liposome-mediated transiently transfection with vectors encoding the empty vector (EV), dominant-negative (KD), or constitutively active (S9A) GSK3β. A Representative micrographs of fluorescent immunocytochemistry staining for HA (red) with nuclear counterstaining with DAPI (blue). Scale bar = 50 μm. B After transfection, cells were treated with 2 ng/ml of TGF-β1 for 24 h. Cell lysates were collected and subjected to immunoblot analysis for E-cadherin, vimentin, FN, PAI-1, CTGF, pH3, HA, and GAPDH. Representative immunoblots were shown.