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. 2021 Apr 30;12(5):432. doi: 10.1038/s41419-021-03709-5

Fig. 7. Profibrogenic plasticity of renal tubules in the mouse model of FA nephropathy is abrogated by TEC-specific GSK3β ablation or by lithium.

Fig. 7

A, B Kidney homogenates were processed for immunoblot analysis for E-cadherin, vimentin, PAI-1, CTGF, and GAPDH. Representative immunoblots were shown. C–F Densitometric analyses of the expression levels of E-cadherin, vimentin, PAI-1, and CTGF, as normalized to the GAPDH expression and expressed as fold changes relative to the control group. *P, **P, ***P < 0.05 (n = 6, ANOVA followed by Tukey’s test). G Representative micrographs of immunofluorescence staining for E-cadherin (green), vimentin (red), and CTGF (red) with nuclear counterstaining with DAPI (blue), or peroxidase immunohistochemistry staining for PAI-1 and pH3 in kidney tissues. Scale bar = 100 μm. H Absolute counting of the number of TEC cells positive for pH3 staining per high power field (HPF). *P, **P, ***P < 0.05 (n = 6, ANOVA followed by Tukey’s test).