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Characteristics of circSP100 during HCMV productive infection. A Northern blot of circSP100. The red arrow indicates the circSP100 RNA transcript; B Subcellular fractionation of circSP100. GAPDH and U2 snRNA were used as the markers for cytoplasm and nuclear fractionation, respectively; C RT-qPCR for the transcript level of circSP100 and linear SP100 mRNA (NM_001080391) in HELFs infected with HCMV at the suggested time points. The data represent the mean ± SD from three replicates.
