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. 2021 Apr 30;12:2482. doi: 10.1038/s41467-021-22750-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Total bone marrow cellularity of two legs from control Con (n = 6), Cux1^+/− (n = 7), Flt3^ITD (n = 6) and Cux1^+/−;Flt3^ITD (n = 6) mice at 10-12 weeks of age. b–e Plots showing absolute numbers of LSK (b), LT-HSC (c), ST-HSC (d) and MPP (e) cells in mice from the indicated genotypes. Representative flow cytometry plots are shown below each bar plot. (f–i) Plots showing absolute numbers of LK (f), CMP (g), GMP (h) and MEP (i) cells in mice from the indicated genotypes. Representative flow cytometry plots are shown below each bar plot. j Percentage of Gr1⁺Mac1⁺ myeloid cells in the bone marrow of mice from the indicated genotypes (n = 6 per group). All plots show mean + s.e.m. Each circle represents one mouse. One-way ANOVA with Tukey’s test for multiple comparisons.