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. 2021 Apr 30;11:9398. doi: 10.1038/s41598-021-88917-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of spikelet-related organs and a mature grain of BTx623. (a) The adaxial view of a secondary branch from the BTx623 panicles. The arrow indicates the second-top spikelet pair. (b) Enlarged view of the second-top spikelet pair. (c) An isolated mature floret from the sessile spikelet. (d) Anthers isolated from the floret. (e) An isolated pistil from the floret. The inset image shows an example of stigma pigmentation values; we scored white as 0 (BTx623), an intermediate pale yellow color as 1 (RIL065), and yellow as 2 (NOG). (f) Front and side views of a mature grain. SS sessile spikelet; PS pedicellate spikelet; PE pedicel; SSL sessile spikelet length; SSW sessile spikelet width; PSL pedicellate spikelet length; PEL pedicel length; AN anther; ANL anther length; STY style; STI stigma; STYL style length; STIL stigma length; STIW stigma width; STIP stigma pigmentation; GRL grain length; GRW grain width; GRT grain thickness. Scale bar = 2 mm in (a, b) and 1 mm in (c–f).