Table 4.

New potential off-target sites in the “corrected” reference genome sequence by genetic variations in the wild-type (WT) plants

Target sgRNA	Off-target sites (NGG)	Off-target sites (NAG)	Off-target sites (NGA)
sgRNA1	0/1	0/7	0/3
sgRNA2	0/3	0/2	0/0
sgRNA3	0/4	0/3	0/12
sgRNA4	0/5	0/11	0/18
sgRNA5	0/4	0/7	0/3
sgRNA6	0/7	0/4	0/4
sgRNA7	0/18	0/13	0/81
sgRNA8	0/5	0/13	0/15

The numbers in front of the ‘/’ symbol represent the off-target variations in Cas9-edited grapevine according to whole-genome sequencing, and the numbers after the ‘/’ symbol represent new potential off-target sites