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. 2021 Apr 30;12(5):433. doi: 10.1038/s41419-021-03719-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Immunoblotting of POU2F2, PDPK1, AKT, p-AKT, mTOR, and p-MTOR in shCtrl, shPOU2F2, and shPOU2F2/PDPK1 cells with or without NSC156529 treatment (20 μM). B Glucose uptake in GBM cells with different treatment. C Glucose consumption in GBM cells with different treatment. D Glycolytic flux changes in GBM cells with different treatment. E Lactate production in GBM cells with different treatment. F Lactate dehydrogenase activity in GBM cells with different treatment. G Immunoblotting of GLUT1, HK2, and PKM2 in shCtrl, shPOU2F2, and shPOU2F2/PDPK1 cells with or without NSC156529 treatment. All data were shown as the mean ± SD, **p < 0.01.