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. 2021 May 1;8:105. doi: 10.1038/s41438-021-00540-z

Fig. 4. Ectopic expression of LcBZR1/2 in Arabidopsis delays floral organ abscission.

Fig. 4

a GUS expression driven by LcBZR1/2 promoters occurred predominantly at the floral organ abscission zone starting from position 5 in Arabidopsis. b Expression levels of LcBZR1/2 in different transgenic lines. LcBZR1/2 driven by the CaMV 35 S promoter was transformed into wild-type Arabidopsis Col. c Photographs of the floral organ abscission process in the Col and LcBZR1/2 transgenic lines. Numbers on the top indicate floral positions along the inflorescence. d BCECF signals of the floral organ AZ of the Col and LcBZR1/2 transgenic lines. The fluorescence images represent merged images of chlorophyll autofluorescence with BCECF fluorescence that were obtained under a confocal laser scanning microscope. The increase in pH is indicated by green fluorescence. The scale bar indicates 200 μm. The images presented for each position and plant are representative images for 3–4 replicates. e pBS of LcBZR1/2 transgenic lines compared with Col (n = 30, bars = SD).