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. 2021 Apr 30;12:2471. doi: 10.1038/s41467-021-22691-2

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Fig. 3 — a Expression of known alpha and gamma markers by skeletal clusters. b Dot plot for the top marker genes for each skeletal cluster, showing more shared expression between clusters S1 and S2. c Expression of novel and specific marker genes for each skeletal MN subtype. d ISH showing expression of novel alpha MN markers Stk32a (red) and Sv2b (yellow) co-expressed with known alpha marker Rbfox3 (magenta) in large diameter Chat+ (cyan) ventral horn neurons. Arrowheads highlight examples of large diameter, triple-positive alpha MNs; arrow highlights the example of a small diameter Chat+ MN that is negative for all 3 alpha markers (i.e., gamma or Type 3). e ISH image overlayed on endogenous Chat-Cre::Ai14 labeling showing expression of S1 marker Gpr149 (yellow), S2 marker Stk32a (cyan), and S3 marker Nrp2 (red). High magnification images highlight a large MN expressing Stk32a and surrounded by C-boutons (examples indicated by arrows), characteristic of alpha MNs, and a small MN expressing Nrp2 and lacking C-boutons, characteristic of gamma MNs. ISH from the ventral horn of lumbar spinal cord sections. Low magnification scale bars, 100 µm. High magnification scale bars, d 50 µm, e 20 µm.