Table 1.

The sensitivity and LOD of the Biomeme and Precision Biomonitoring SARS-CoV-2 tests.

(A)
SARS-CoV-2	Biomeme SARS-CoV-2 test		SARS-CoV-2 (estimated copies per reaction)	Precision Biomonitoring TripleLock SARS-CoV-2 test
	Target	Ct value		Target	Ct value
Undiluted	Orf1ab gene	11.24	15 × 108	5′UTR gene	11.40
	S gene	10.13		E gene	10.24
10–1	Orf1ab gene	14.31	15 × 107	5′UTR gene	14.40
	S gene	14.48		E gene	12.92
10–2	Orf1ab gene	17.29	15 × 106	5′UTR gene	17.68
	S gene	17.45		E gene	16.06
10–3	Orf1ab gene	21.31	15 × 105	5′UTR gene	21.01
	S gene	21.48		E gene	19.47
10–4	Orf1ab gene	24.24	15 × 104	5′UTR gene	24.28
	S gene	24.43		E gene	22.47
10–5	Orf1ab gene	27.96	15 × 103	5′UTR gene	27.76
	S gene	28.12		E gene	26.26
10–6	Orf1ab gene	31.39	15 × 102	5′UTR gene	31.39
	S gene	30.99		E gene	29.23
10–7	Orf1ab gene	32.65	15 × 101	5′UTR gene	33.45
	S gene	33.99		E gene	32.63
10–8	Orf1ab gene	34.69	15 × 100	5′UTR gene	35.76
	S gene	33.82		E gene	34.14
10–9	Orf1ab gene	–	15 × 10–1	5′UTR gene	–
	S gene	–		E gene	–

(B)
SARS-CoV-2	Target	Ct value
		Biomeme	E_Sarbeco
Undiluted	Orf1ab gene	9.47	13.36
	S gene	9.84
10–1	Orf1ab gene	12.09	16.47
	S gene	12.31
10–2	Orf1ab gene	16.73	21.30
	S gene	16.94
10–3	Orf1ab gene	20.49	25.00
	S gene	20.61
10–4	Orf1ab gene	24.78	28.66
	S gene	24.61
10–5	Orf1ab gene	28.36	31.99
	S gene	27.58
10–6	Orf1ab gene	31.78	34.89
	S gene	30.87
10–7	Orf1ab gene	32.92	37.14
	S gene	32.91
NTC	Orf1ab gene	0.00	0.00
	S gene	0.00

The M1 Sample Prep Cartridge Kit for RNA 2.0 was used to extract RNA from serial dilutions of cell culture-amplified SARS-CoV-2. Following the extractions, 20 µl of RNA from each dilution was used to set up the Biomeme and Precision Biomonitoring SARS-CoV-2 Test on Franklin three9 (A). Because the same extracted RNA from the dilutions were used for both assays, there was no spiking of RPC during extraction as required by the Biomeme assay, this was done to avoid interfering with the E gene of the Precision Biomitoring assay as both RPC and E gene are on the same channel (TexasRedX) on Franklin three9; as a result, all internal controls were excluded from the runs and analysis. In addition, the detection of the full SARS-CoV-2 targets (even in the absence of the internal controls) in both assays means that the tests are positive/valid. In another experiment, MagMAX™ CORE Nucleic Acid Purification Kit-extracted RNA from cell culture-amplified SARS-CoV-2 dilutions was used to set up Biomeme and E_Sarbeco SARS-CoV-2 assays to compare their sensitivities. The Biomeme SARS-CoV-2 assay was run on Franklin three9 while the E_Sarbeco assay was run on ABI 7500 (B).