Table 5.
Evaluation of clinical performance Biomeme and Precision Biomonitoring TripleLock SARS-CoV-2 assays with hamster samples.
| (A) | ||||
|---|---|---|---|---|
| Hamster ID | Target (Biomeme) | Ct value | ||
| Nasal wash | Rectal swab | Oral swab | ||
| 81 | Orf1ab gene | 14.42 | 24.73 | 32.08 |
| S gene | 14.55 | 24.83 | 29.68 | |
| RPC | No Ct* | 23.83 | 24.45 | |
| 82 | Orf1ab gene | 12.87 | 28.25 | 22.21 |
| S gene | 12.88 | 29.08 | 21.12 | |
| RPC | No Ct* | 27.46 | 28.19 | |
| 83 | Orf1ab gene | 16.09 | 26.99 | 22.59 |
| S gene | 16.23 | 26.33 | 21.42 | |
| RPC | No Ct* | 25.04 | 26.43 | |
| 84 | Orf1ab gene | 13.29 | 25.73 | 20.85 |
| S gene | 13.65 | 23.17 | 20.92 | |
| RPC | No Ct* | 24.84 | No Ct* | |
| 85 | Orf1ab gene | 13.63 | 23.86 | 19.88 |
| S gene | 14.05 | 23.26 | 19.89 | |
| RPC | No Ct* | 23.31 | 23.28 | |
| 86 | Orf1ab gene | 15.61 | 24.69 | 20.86 |
| S gene | 16.03 | 23.22 | 20.12 | |
| RPC | No Ct* | 23.15 | 19.76 | |
| Uninfected_1 | Orf1ab gene | – | No Ct | No Ct |
| S gene | – | No Ct | No Ct | |
| RPC | – | 28.64 | 29.51 | |
| Uninfected_2 | Orf1ab gene | – | No Ct | No Ct |
| S gene | – | No Ct | No Ct | |
| RPC | – | 28.85 | 29.13 | |
| Uninfected_3 | Orf1ab gene | – | No Ct | No Ct |
| S gene | – | No Ct | No Ct | |
| RPC | – | 29.02 | 30.07 | |
| (B) | ||||
|---|---|---|---|---|
| Hamster ID | Target (PBI TripleLock) | Ct value | ||
| Nasal wash | Rectal swab | Oral swab | ||
| 81 | 5′UTR gene | 16.02 | 31.49 | – |
| E_gene | 13.22 | 26.21 | – | |
| RNaseP | No Ct* | 39.23 | – | |
| 82 | 5′UTR gene | 16.10 | 29.65 | 23.84 |
| E_gene | 13.15 | 26.38 | 20.21 | |
| RNaseP | No Ct* | No Ct* | No Ct* | |
| 83 | 5′UTR gene | 17.73 | 30.62 | 23.09 |
| E_gene | 14.36 | 26.26 | 19.27 | |
| RNaseP | No Ct* | No Ct* | 39.81 | |
| 84 | 5′UTR gene | 16.13 | 30.03 | 22.42 |
| E_gene | 11.91 | 26.50 | 18.17 | |
| RNaseP | 36.33 | 42.40 | 35.06 | |
| 85 | 5′UTR gene | 15.72 | 25.84 | 22.99 |
| E_gene | 13.07 | 23.76 | 19.18 | |
| RNaseP | No Ct* | 33.97 | No Ct* | |
| 86 | 5′UTR gene | 18.36 | 23.29 | 23.39 |
| E_gene | 15.09 | 22.28 | 20.95 | |
| RNaseP | No Ct* | 31.26 | 32.24 | |
| Uninfected_1 | 5′UTR gene | – | No Ct | No Ct |
| E_gene | – | No Ct | No Ct | |
| RNaseP | – | 39.65 | No Ct | |
| Uninfected_2 | 5′UTR gene | – | No Ct | No Ct |
| E_gene | – | No Ct | No Ct | |
| RNaseP | – | 35.07 | 35.17 | |
| Uninfected_3 | 5′UTR gene | – | No Ct | No Ct |
| E_gene | – | No Ct | No Ct | |
| RNaseP | – | 35.24 | No Ct | |
The M1 Sample Prep Cartridge Kit for RNA 2.0 was used to extract RNA from the nasal wash, rectal swab, and oral swab samples from SARS-CoV-2-inoculated hamster on days 2 (#81–83) and 5 (#84–86) post-inoculation. Following RNA extraction, the extracts were tested with Biomeme (A) and Precision Biomonitoring TripleLock SARS-CoV-2 (B) assays on Franklin three9.
*The RNA Process Control may not amplify (No Ct) when the two SARS-CoV-2 gene signals are very strong.