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. 2021 Apr 30;7:88. doi: 10.1038/s41420-021-00470-8

Fig. 4. HBx transfection induces liver cancer cell growth and suppresses SIRT4 expression.

Fig. 4

A Western blot analysis confirms HBx protein expression in HBV negative cancer cell lines HepG2 and Huh7 transfected with HBx expression plasmid. B and C HBx transfection induces cell growth and proliferation in HepG2 and Huh7 cells. D HBx transfection induces colony formation in HepG2 and Huh7 cells transfected with HBx expression plasmid or control empty vector. The number of colonies was counted from five randomly selected views in cells transfected with HBx expression plasmid relative to the control cells transfected with empty vector. E qRT-PCR and Western blot analysis of SIRT4 expression levels in cells with stable HBx plasmid transfection. All error bars show the standard error of the mean (SEM). *p < 0.05, **p < 0.01, and ***p < 0.001.