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. 2021 Apr 30;7:88. doi: 10.1038/s41420-021-00470-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Increased caspase3/7 activity in cells after overexpression of SIRT4. Measurement of relative luminescence units (RLU) to control cells. B Representative black and white images showing SIRT4 overexpression induces senescence in HepG2-HBx and HepG2.215 cells with the morphological changes indicated by black arrows compared to their dividing cell counterparts. C Fluorescence staining of senescent cells with enlarged size and flattened shape as indicated by white arrows. D Representative images of SIRT4 overexpression cells show positive staining for SA-β-gal activity. Control cells show little or no staining. E The percentage of SA-β-gal positive cells were counted from five randomly selected views in cells transfected with SIRT4 expression plasmid relative to the control cells transfected with empty vector. Data are presented as mean ± SD of triplicate samples. **p < 0.001, and ***p < 0.0001.