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. 2021 Apr 30;7:88. doi: 10.1038/s41420-021-00470-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A and B Flow cytometry analysis of HepG2-HBx and HepG2.2.15 cells after transfection with SIRT4 plasmid for 24, 48, and 72 h, followed by staining with annexin V-FITC and PI. The percentage of apoptotic cells was increased with time. C and D Flow cytometry analysis of cell cycle distribution indicating SIRT4 overexpression induces cell-cycle arrest at G2/M. E Western blot-analysis of cell-cycle-related proteins expression at 48 h after SIRT4 transfection. F Representative fluorescence images showing the retention of Cyclin B1 in the cytoplasm after SIRT4 overexpression. The quantitative data shown are mean ± SEM of triplicate samples. *p < 0.01 and ***p < 0.0001.