FIGURE 5.

USP35 modulates cell growth, colony formation, tumor progression, and ferroptosis in EGFR mutated H1650 cells. A, Relative USP35 mRNA level in H1650 cells with or without USP35 silence (n = 6). B, Cell viability and colony formation (n = 6). C, Tumor volumes and tumor weights at day 25 after cell inoculation (n = 6). D, Lipid ROS generation and MDA levels in H1650 cells with or without USP35 silence (n = 6). E, GSH levels and GPX4 activities in H1650 cells with or without USP35 silence (n = 6). F, Relative LIP and Fe²⁺ levels (n = 6). G, Relative USP35 mRNA level in H1650 cells with or without USP35 overexpression (n = 6). H,I, Cell viability and colony formation (n = 6). J, Tumor volumes and tumor weights at day 25 after cell inoculation (n = 6). K,L, Lipid ROS generation and MDA levels in H1650 cells (n = 6). M, Relative LIP and Fe²⁺ levels (n = 6). Data are shown as mean ± SD, *P < .05 versus the matched group. NS indicates no significance