Fig. 4.

The effect of S1PRs on adipocyte-induced ovarian cancer cell proliferation. a SKOV3 cells were transfected with control siRNA or specific siRNA targeting S1PR1–3 as indicated. After 24 h of transfection, the mRNA levels of S1PR1–3 were determined by quantitative RT-PCR. The data are expressed as the change with respect to control siRNA. b After 48 h of transfection, the protein levels of S1PR1–3 were determined by western blot analysis and normalized to GAPDH. c SiRNA-transfected cells were cultured with SFM or Adi-CM for 48 h. Cell proliferation was measured by CCK-8 assay. d to f Cells were transfected with the indicated siRNAs and cultured with SFM or Adi-CM for 24 h. Levels of total and phosphorylated ERK (pERK) were then determined by western blot analysis. The data are presented as the means±SD. *, P < 0.05