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. 2021 Apr 30;19:89. doi: 10.1186/s12915-021-01021-4

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Genomic distribution of sex-biased genes in M. longipes. a Percentage of male-biased, female-biased and unbiased genes (from top to bottom) in the X chromosome and the autosomes across the three pairs of legs. Biased-distribution of the sex-biased genes on the X chromosome was estimated using Fisher’s exact tests: *p value< 0.05. b Large genomic clusters of sex-biased genes along scaffold #2 and the scaffold #8. Clusters highlighted in blue represent genomic regions enriched in male-biased genes. Clusters highlighted in purple represent genomic regions enriched in female-biased genes. Solid red frame indicates genomic clusters enriched in male- and female-biased genes specifically in the third legs. Dotted red frame indicates genomic clusters enriched in male-biased genes in all three legs but with different degrees of fold-change, recapitulating the degree of leg length exaggeration. c Genomic clusters of consecutive male- (blue) or female-biased (purple) genes in the three pairs of legs. Cluster size indicates the number of consecutive genes. Note that the y axis is log scaled. Error bars contain 95% of randomly generated bootstrap values