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. 2021 Feb 23;41(3):e00542-20. doi: 10.1128/MCB.00542-20

TABLE 2.

Target sequences of sgRNAs for CRISPR-Cas9 gene knockout

Primer name Sequence (5′3′)
IKKα-targeting sgRNA, forward CACCGGTACCAAAAACAGAGAACGA
IKKα-targeting sgRNA, reverse AAACTCGTTCTCTGTTTTTGGTACC
IKKβ-targeting sgRNA, forward CACCGTCAGCCCCCGGAACCGAGAG
IKKβ-targeting sgRNA, reverse AAACCTCTCGGTTCCGGGGGCTGAC
TBK1-targeting sgRNA, forward CACCGAAATATCATGCGTGTTATAG
TBK1-targeting sgRNA, reverse AAACCTATAACACGCATGATATTTC
NEMO-targeting sgRNA, forward CACCGGCTGCACCTGCCTTCAGAACA
NEMO-targeting sgRNA, reverse AAACTGTTCTGAAGGCAGGTGCAGCC