FIG 7.

Proposed model. During interphase, cytoplasmic unphosphorylated PHLPP1 functions to regulate substrates such as Akt (left). PHLPP1 is in close proximity to membrane-associated scaffolds such as Scrib. Upon entry into mitosis, the PHLPP1 NTE is hyperphosphorylated by Cdk1 (right). Additionally, a diminished interaction with Scrib and an enhanced interaction with mitotic scaffolds are observed. Deletion of PHLPP1 results in mitotic defects, suggesting that this pathway is important for proper mitotic progression.