FIG 2.

Germ line truncating mutants G522Efs and R347X cause low bone mass and narrow bones. (A) Gross body weights of 10-week-old male Recql4^+/+, Recql4^G522Efs/+, and Recql4^R347X/+ mice. (B to D) Micro-CT measurements of (B) tibial length, (C) mediolateral width, and (D) anteroposterior width from 10-week-old male Recql4^+/+, Recql4^K525A/K525A, Recql4^G522Efs/+, and Recql4^R347X/+ mice. (E) Trabecular region of interest beginning at 3.5% (offset) distal to the growth plate and extending for 5% (ROI) of the total tibia length. (F) Trabecular bone volume. (G) Trabecular number. (H) Trabecular separation. (I) Representative images (axial plane) of reconstructed trabecular bone with color-coded quantitative mineralization from germ line Recql4 mutants. (J) Cortical region of interest beginning at 40% (offset) distal to the growth plate and extending for 5% (ROI) of the total tibia length. (K) Cortical thickness. (L) Periosteal perimeter. (M) Moment of inertia. (N) Representative images (axial plane) of reconstructed cortical bone with color-coded quantitative mineralization from germ line Recql4 mutants. Data expressed as mean ± SEM; ordinary one-way analysis of variance (ANOVA). *, P < 0.05; **, P < 0.01; ****, P < 0.0001. +/+, n = 7; K/K, n = 10; G/+, n = 6, R/+, n = 7. Experiments were independently executed on separate cohorts, with results pooled for presentation. K, K525A; G, G522Efs; R, R347X.