FIG 7.

Slits act as repellent factors to influence β-cell migration. (A) Schematic diagram of Transwell cell migration assay. INS-1 cells were seeded in cell culture inserts over INS-1 conditioned media, fresh culture media (n = 9), or INS-1 conditioned media supplemented with 2.5 µg of each recombinant SLIT protein (n = 6). (B) Results of cell migration assay. The average number of cells migrating per field of view is plotted. ****, P < 0.0001 by ANOVA with Tukey-Kramer post hoc tests. (C) Representative images of a single field of view of a cell migration insert used in the experiments shown in panel B.