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. Author manuscript; available in PMC: 2021 Oct 14.
Published in final edited form as: J Am Chem Soc. 2020 Oct 5;142(41):17515–17523. doi: 10.1021/jacs.0c07245

Figure 2: AggFluor P1 harbors a rotational barrier that could explain its fluorescence activation with misfolded oligomers of lower viscosities.

Figure 2:

a) Structure of P1. b) Measurement of viscosity sensitivity (x) using the linear fitting of double logarithmic plots of intensity versus viscosity using ethylene glycol/glycerol mixtures, P1 shows a low viscosity sensitivity x = 0.28. c) Fluorescent intensity of P1 increase by 1.5-fold from glycerol (972 cp) to glycerol at −80°C (3•1011 cp) d). Diagram showing the donor and acceptor of P1 upon excitation. TICT state is formed via rotation between donor and acceptor along the dihedral angle φ. e) SA-CASSCF rigid scan over angle φ identifies the excited state rotational barrier at 45° with Ea = 0.36 eV. f) TICT initiates at rotational angles greater than 45°.