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. 2021 May 1;20:94. doi: 10.1186/s12934-021-01583-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Codon optimization reduces the N-terminal truncation of P[4]. a Truncation variants of the original P[4] sequence by LC–MS. The N-terminus of each truncation variant is indicated on the antigen diagram and predicted RNA hairpin structure. b Relative abundance within the sample of truncated P[4] variants identified by LC–MS (top). Free energies of RNA hairpin formation after codon optimization for each variant (bottom). c SDS-PAGE of variants of P[4]