Figure 5.

IL-6 activated the mitogen-activated protein kinase (MAPK) signaling pathway in MDSCs. (a, b) Heat map of RNA sequencing results to evaluate differentially expressed genes in MDSCs with or without IL-6 treatment. (c, d) Differentially expressed genes were enriched for several signaling pathways. (e, f) Phosphorylation of critical proteins in the MAPK signaling pathway (MEK2, ERK, and MNK1) was evaluated by western blotting. (g–i) Intensity ratio of each band. Mean ± SD, ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.005.