Figure 6.

IL-6 promoted MDSC proliferation and immune-suppressive effects by activating the MAPK signaling pathway. (a) CCK-8 assays were used to evaluate MDSCs with or without IL-6 and/or aloesin treatment. (b) MDSC proliferation rates were evaluated according to fluorescence attenuation after IL-6 and/or aloesin treatment. (c–f) RT-PCR was used to evaluate Arg1 and iNOS levels in MDSCs with or without IL-6 and/or aloesin treatment. (g–j) Summary of the suppressive effect of MDSCs by coculture with CD8⁺ T cells and CD4⁺ T cells at 1 : 4 ratios. Mean ± SD, ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.005.