Fig. 1. IMD1-53 alleviated atherosclerotic lesions at the aortic root of ApoE−/− mice.
Eight-week-old male ApoE−/− were fed a standard chow diet (con) or a high-fat diet (HFD) for 16 weeks. After 10 weeks of HFD feeding, ApoE–/– mice received either PBS or intermedin1-53 (IMD1-53) during the left 6 weeks of high-fat diet feeding. A Gross view of en face-prepared aortas stained with Oil Red O. The red dots are lesions stained positively for Oil Red O. Yellow arrows pointed to the Oil red O-stained lesions. B Representative image of Oil red O-stained aortic root lesions. Scale bars, 500 μm. C Quantification of Oil red O positive area. D Representative image of H&E-stained aortic root lesion. Black arrows indicate the atherosclerotic lesions. Scale bars, 500 μm. E Quantification of aortic root lesion area. n = 7–11. Data are mean ± SD. *P < 0.05, **P < 0.01; one-way ANOVA.