FIGURE 5.

Immunomodulatory effects of MSCs in the intestine. (A) Density plots of the gating strategy for CD8⁺ T cells among small‐intestinal intraepithelial lymphocytes (IELs) and Peyer's patches (PPs) of ALI mice by flow cytometry. (B) Total numbers of IELs and PPs in mice with acute lung injury mice. Each symbol indicates an individual mouse (mean ± SEM, n = 6). (C) Flow cytometric analyses of CD8⁺T cells among IELs and PPs from the indicated mice. (D) Frequency of CD3⁺ T, CD4⁺ T, CD8⁺ T cells and F4/80⁺CD11b⁺ cells in IELs in the PBS, LPS/PBS and LPS/MSC groups. A decrease in the percentage of CD8ααTCRγδ⁺ T cells was observed in the LPS/MSC group at days 1 and 3. Each symbol indicates an individual mouse (mean ± SEM, n = 6). *P <.05, **P <.01, ***P <.001 by Student's t‐test. (E) The frequency of T cells in PPs at days 1, 3 and 7 in PBS, LPS/PBS and LPS/MSC groups. Each symbol indicates an individual mouse. (mean ± SEM, n = 6). **P <.01, ***P <.001 by Student's t‐test