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. 2021 Mar 23;54(5):e13026. doi: 10.1111/cpr.13026

FIGURE 2.

FIGURE 2

Overexpression of miR‐378a in ECs promotes cell proliferation, migration and angiogenesis. (A) The proliferation of ECs treated with PBS, mimic‐NC and miR‐378a mimic was tested by CCK‐8 assay (n = 3). (B) The migration of ECs treated with PBS, mimic‐NC and miR‐378a mimic was detected by transwell assay (scale bar, 100 μm). (C) Quantitative analysis of the migrated cells in B (n = 5). (D) The migration of ECs treated with PBS, mimic‐NC and miR‐378a mimic was measured by scratch wound assay (scale bar, 200 μm). (E) Quantitative analysis of the migration rates in D (n = 5). (F) Representative images of the tube formation assay in ECs treated with PBS, mimic‐NC and miR‐378a mimic (scale bar, 200 μm). (G) Quantitative analyses of the total branching points and total tube length in F (n = 3). (H) Detection of the protein level of VEGF in ECs by Western blot. (I) Quantitative analysis of the relative protein expression in H (n = 3). *P <.05, **P <.01 vs. the Control group; # P <.05, ## P <.01 vs. the mimic‐NC group