FIGURE 3.

Identification of Sufu as a downstream target gene of miR‐378a. (A) The relative luciferase activity of luciferase reports with wild‐type or mutant Sufu was determined in ECs, which were transfected with mimic‐NC or miR‐378a mimic (n = 3). (B) The mRNA expression of Sufu in ECs after transfection with mimic‐NC, miR‐378a mimic, inhibitor‐NC and miR‐378a inhibitor was determined by qRT‐PCR (n = 3). (C) The protein expression of Sufu in ECs detected using Western blot. (D) Quantitative analysis of the relative protein expression in C (n = 3). *P <.05, **P <.01 vs. the mimic‐NC group; ^# P <.05, ^## P <.01 vs. the inhibitor‐NC group