FIG 5.

Effects of pH on V. cholerae flagellar motility. (A) Distributions of diffusion coefficient of Classical O395 from single-cell trajectories in spent medium (Spent) or in fresh medium at different pHs. Trajectories below 10 μm²/s were categorized as nonmotile and excluded from the remaining analyses. (B) Distributions of swimming speed from the motile cell populations. (C) Distributions of trajectory directional persistence from the motile cell populations. (D) Distributions of diffusion coefficients of El Tor C6706 from single-cell trajectories in spent medium (Spent) or in fresh medium at different pHs. An mshA mutant was also tracked. (E) Distributions of swimming speed from the motile cell populations. (F) Distributions of trajectory directional persistence from the motile cell populations. Each distribution represents 3 replicates combining between 2,000 and 10,000 individual trajectories (between 100 and 500 min of cumulative time). Circles indicate means for the motile populations.