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. 2021 May 1;20:93. doi: 10.1186/s12934-021-01582-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Functional surface display of IsPETase variants comparing a C-IgAP and LppOmpA anchor module. a Whole-cell and in-gel fluorescence for IsPETase variants (wt: wild type; A: Austin, S: Son) surface-displayed using either C-IgAP or LppOmpA module with and without L-rhamnose induction. The nanobody:GFP binding workflow is indicated. Unbound GFP was run on the outer left lane. Samples have been normalized to ODU. b Absorbance change at 405 nm measured on pNP-acetate for IsPETase variants expressed via the two modules. Active PETase degrades pNP-acetate to pNP, and acetic acid as illustrated. NC: Negative control BL21(DE3) cells not harboring the expression vector. Statistical evaluation: Multiple comparison analysis (two-way ANOVA); ns: no significant statistical difference, ****: p-value < 0.0001