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. 2021 May 1;19:124. doi: 10.1186/s12951-021-00865-w

Fig. 4.

Fig. 4

Fig. 4

Mechanism of combination aPD-L1 with CA4-NPs. Immunofluorescence staining for a CD31, b HIF-1α, c PD-L1 expression of tumor tissues 48 h post-treatment and quantitative immunofluorescence analysis. CA4-NPs (20) represented that the dose of CA4-NPs was 20 mg kg−1 and CA4-NPs (40) represented that the dose of CA4-NPs was 40 mg kg−1. Scale bar: 20 μm. Significance between each pair of groups was calculated using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The values are presented as the mean ± SD, n = 3