Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 1;19:124. doi: 10.1186/s12951-021-00865-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — a Ki67 and TUNEL staining of tumor tissues after different treatments; b Immunohistochemical reactivity for blood vessel endothelial cell marker CD31 in subcutaneous Hepa1-6 bearing mice; c Double-color immunofluorescence staining for CD4 (Red) and CD8(Green) expression of tumor tissues, and quantitative immunofluorescence analysis; d The secretions of cytokines in serum and tumor site were measured by ELISA (levels of IFN-γ and IL-4); e Immunofluorescence staining for LAG-3 and TIM-3 expression of tumor tissues, and quantitative immunofluorescence analysis. Black scale bar = 100 μm and white scale bar = 20 μm. Significance between each pair of groups was calculated using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The values are presented as the mean ± SD, n = 3

Fig. 6 — a Ki67 and TUNEL staining of tumor tissues after different treatments; b Immunohistochemical reactivity for blood vessel endothelial cell marker CD31 in subcutaneous Hepa1-6 bearing mice; c Double-color immunofluorescence staining for CD4 (Red) and CD8(Green) expression of tumor tissues, and quantitative immunofluorescence analysis; d The secretions of cytokines in serum and tumor site were measured by ELISA (levels of IFN-γ and IL-4); e Immunofluorescence staining for LAG-3 and TIM-3 expression of tumor tissues, and quantitative immunofluorescence analysis. Black scale bar = 100 μm and white scale bar = 20 μm. Significance between each pair of groups was calculated using Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The values are presented as the mean ± SD, n = 3