Table 1.

Margaritifera margaritifera genome assembly, read alignment, gene prediction, and annotation general statistics

	Contiga	Scaffolda
Total number of sequences (≥1,000 bp)	265,718	105,185
Total number of sequences (≥10,000 bp)	66,019	15,384
Total number of sequences (≥25,000 bp)	18,725	11,583
Total number of sequences (≥50,000 bp)	4,284	9,265
Total length (≥1,000 bp)	2,230,001,992	2,472,078,101
Total length (≥10,000 bp)	1,523,143,239	2,293,496,118
Total length (≥25,000 bp)	789,559,702	2,236,013,546
Total length (≥50,000 bp)	299,796,296	2,152,307,394
N50 length (bp)	16,899	288,726
L50	34,910	2,393
Maximum length (bp)	209,744	2,510,869
GC content, %	35.42	35.42
Clean paired-end (PE) reads alignment stats
Pecentage of mapped PE (%)	—	97.754
Pecentage of proper pairs PE (%)	—	90.653
Average PE sequence coverage	—	181.968
Pecentage of scaffolds with any coverage (%)	—	100.00
Total BUSCOS for the genome assembly (%)
#Euk database	—	C: 86.8% (S: 85.8%, D: 1.0%), F: 5.9%
#Met database	—	C: 84.9% (S: 83.8%, D: 1.1%), F: 4.9%
Gene prediction and annotation statsb
Protein-coding genes (CDS)	—	35,119
Transcripts (mRNA)	—	40,544
Protein-coding genes functional annotated	—	26,836
Transcripts functional annotated	—	31,584
Total gene length (bp)	—	902,994,752
Total mRNA length (bp)	—	1,101,526,909
Total CDS length (bp)	—	52,211,391
Total exon length (bp)	—	52,211,391
Total intron length (bp)	—	1,024,450,311
Total BUSCOS for the predicted proteins (%)
+Euk database	—	C: 90.6% (S: 81.2%, D: 9.4%), F: 3.9%
+Met database	—	C: 92.6% (S: 82.3%, D: 10.3%), F: 3.2%

C: complete; S: single; D: duplicated; F: fragmented.

#Euk: from a total of 303 genes of Eukaryota library profile.

#Met: from a total of 978 genes of Metazoa library profile.

⁺Euk: from a total of 255 genes of Eukaryota library profile.

⁺Met: from a total of 954 genes of Metazoa library profile.

^aAll statistics are based on contigs/scaffolds of size ≥1,000 bp.

^bAll statistics are based on contigs/scaffolds of size ≥2,500 bp.